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Modelling the growth and ethanol production of Brettanomyces bruxellensis at different glucose concentrations
Author(s) -
AguilarUscanga M.G.,
GarciaAlvarado Y.,
GomezRodriguez J.,
Phister T.,
Delia M.L.,
Strehaiano P.
Publication year - 2011
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2011.03081.x
Subject(s) - yeast , biomass (ecology) , ethanol fuel , fermentation , substrate (aquarium) , ethanol , chemistry , food science , biochemistry , biology , ecology
Aim: To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. Methods and Results: A Matlab algorithm was developed for the estimation of model parameters. Glucose fermentation by B. bruxellensis was studied by varying its concentration (5, 9·3, 13·8, 16·5, 17·6 and 21·4%). The increase in substrate concentration up to a certain limit was accompanied by an increase in ethanol and biomass production; at a substrate concentration of 50–138 g l −1 , the ethanol and biomass production were 24, 59 and 6·3, 11·4 g l −1 , respectively. However, an increase in glucose concentration to 165 g l −1 led to a drastic decrease in product formation and substrate utilization. Conclusions: The model successfully simulated the batch kinetic observed in all cases. The confidence intervals were also estimated at each phase at a 0·95 probability level in a t‐Student distribution for f degrees of freedom. The maximum ethanol and biomass yields were obtained with an initial glucose concentration of 138 g l −1 . Significance and Impact of the Study: These experiments illustrate the importance of using a mathematical model applied to kinetic behaviour on glucose concentration by B. bruxellensis .