Premium
Identification and evaluation of an outer membrane protein OmpU from a pathogenic Vibrio harveyi isolate as vaccine candidate in turbot ( Scophthalmus maximus )
Author(s) -
Wang Q.,
Chen J.,
Liu R.,
Jia J.
Publication year - 2011
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2011.03062.x
Subject(s) - turbot , biology , vibrio harveyi , microbiology and biotechnology , scophthalmus , plasmid , dna vaccination , recombinant dna , virology , vibrionaceae , gene , vibrio , genetics , bacteria , fishery , fish <actinopterygii>
Aims: The main aims of this study were to clone and express a new outer membrane protein U (OmpU) from a pathogenic Vibrio harveyi SF‐1 and investigate its immune efficiency as a vaccine candidate against V. harveyi infection in turbot ( Scophthalmus maximus ). Methods and Results: In this study, a new gene, ompU was cloned from the genomic DNA of pathogenic V. harveyi SF‐1. The ompU gene encoded a 35 kDa protein, which was purified by Ni‐NTA His‐Bind Resin column. A DNA vaccine was constructed by inserting ompU gene into pEGFP‐N1 plasmid. Turbot were injected intramuscularly with the purified OmpU protein and the recombinant pEGFP‐N1/ ompU plasmid, respectively. The fish vaccinated with the purified OmpU protein were completely protected with a relative per cent of survival (RPS) of 100% against pathogenic V. harveyi infection. Efficient protection was also found in the pEGFP‐N1/ ompU vaccinated group, with a RPS of 51·4%. Significant specific antibody responses were detected in the vaccinated turbot by indirect enzyme‐linked immunosorbent assay. Conclusions: A new OmpU was cloned and expressed. Both OmpU protein vaccine and DNA vaccine showed good immune protections in turbot. Significance and Impact of the Study: The OmpU was identified to be a new effective vaccine candidate and could be used as subunit vaccine and DNA vaccine for disease control caused by pathogenic V. harveyi .