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Intra‐laboratory validation of the Ridascreen ® SET Total kit for detecting staphylococcal enterotoxins SEA to SEE in cheese
Author(s) -
Ostyn A.,
Guillier F.,
Prufer A.L.,
Papinaud I.,
Messio S.,
Krys S.,
Lombard B.,
Hennekinne J.A.
Publication year - 2011
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2011.03025.x
Subject(s) - microbiology and biotechnology , enterotoxin , biology , genetics , escherichia coli , gene
Aim: To determine the performance of the Ridascreen ® SET Total kit, after sample extraction and concentration by dialysis, with regard to its use in official controls for staphylococcal enterotoxins under European Regulation (EC) No. 2073/2005 modified. This study was conducted on naturally contaminated cheese samples and compared with the results of the previously validated Vidas ® SET2 kit. Methods and Results: The effectiveness of the Ridascreen ® SET Total kit on naturally contaminated cheeses was compared to that of the Vidas ® SET2 kit by applying the EN ISO 16140 standard. Sensitivity and specificity were also compared using spiked buffer solutions and cheese samples with SEA to SEE toxins. Conclusions: This study showed that the Ridascreen ® SET Total kit is as effective as the Vidas ® SET2 kit. Significance and Impact of the Study: The Ridascreen ® SET Total kit was found to specifically detect SEA to SEE in cheeses. The Ridascreen ® SET Total can therefore be used to check the staphylococcal enterotoxin content and ensure consumer protection.