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Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two‐stage oxygen supply control strategy based on oxygen transfer coefficient
Author(s) -
Qu L.,
Ji X.J.,
Ren L.J.,
Nie Z.K.,
Feng Y.,
Wu W.J.,
Ouyang P.K.,
Huang H.
Publication year - 2011
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2010.02960.x
Subject(s) - docosahexaenoic acid , fermentation , productivity , oxygen evolution , food science , yield (engineering) , biology , biochemistry , oxygen , chemistry , fatty acid , polyunsaturated fatty acid , electrochemistry , macroeconomics , materials science , electrode , metallurgy , economics , organic chemistry
Aims: To improve the yield and productivity of docosahexaenoic acid (DHA) by Schizochytrium sp. in terms of the analysis of microbial physiology. Methods and Results: A two‐stage oxygen supply control strategy, aimed at achieving high concentration and high productivity of DHA, was proposed. At the first 40 h, K L a was controlled at 150·1 h −1 to obtain high μ for cell growth, subsequently K L a was controlled at 88·5 h −1 to maintain high q p for high DHA accumulation. Finally, the maximum lipid, DHA content and DHA productivity reached 46·6, 17·7 g l −1 and 111 mg l −1 h −1 , which were 43·83%, 63·88% and 32·14% over the best results controlled by constant K L a. Conclusions: This paper described a two‐stage oxygen supply control strategy based on the kinetic analysis for efficient DHA fermentation by Schizochytrium sp. Significance and Impact of the study: This study showed the advantage of two‐stage control strategy in terms of microbial physiology. As K L a is a scaling‐up parameter, the idea developed in this paper could be scaled‐up to industrial process and applied to other industrial biotechnological processes to achieve both high product concentration and high productivity.