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The ability of nisin F to control Staphylococcus aureus infection in the peritoneal cavity, as studied in mice
Author(s) -
Brand A.M.,
De Kwaadsteniet M.,
Dicks L.M.T.
Publication year - 2010
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2010.02948.x
Subject(s) - staphylococcus aureus , nisin , microbiology and biotechnology , peritoneal cavity , in vivo , micrococcaceae , bacteriocin , biology , chemistry , bacteria , antibacterial agent , antibiotics , antimicrobial , anatomy , genetics
Aims: To determine the ability of nisin F to control systematic infection caused by Staphylococcus aureus , using C57BL/6 mice as a model. Methods and Results: Twelve mice were intraperitoneally injected with 1 × 10 8 viable cells of Staph. aureus Xen 36 containing the modified Photorhabdus luminescence luxABCDE operon on plasmid pAUL‐A Tn 4001 . After 4 h, six mice were intraperitoneally injected with 640 arbitrary units (AU) nisin F, and six were injected with sterile saline. Six mice, not infected with Staph. aureus , were treated with nisin F, and six not infected were left untreated. The viability of Staph. aureus Xen 36 was monitored over 48 h by recording photon emission levels. Nisin F suppressed Staph. aureus for 15 min in vivo . No abnormalities were recorded in blood analyses and internal organs of mice treated with nisin F. Conclusions: Nisin F suppressed the growth of Staph. aureus in the peritoneal cavity for at least 15 min. Re‐emergence of Staph. aureus bioluminescence over the next 44 h suggests that nisin F was inactivated, most probably by proteolytic enzymes. Significance and Impact of the Study: A single dosage of nisin F administered in the peritoneal cavity controlled the growth of Staph. aureus for at least 15 min in vivo .