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Characterization of Shiga toxin‐producing Escherichia coli isolated from dairy cows in Argentina
Author(s) -
Fernández D.,
Irino K.,
Sanz M.E.,
Padola N.L.,
Parma A.E.
Publication year - 2010
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2010.02904.x
Subject(s) - stx2 , serotype , escherichia coli , biology , microbiology and biotechnology , shiga toxin , virulence , multiplex polymerase chain reaction , population , dairy cattle , virology , veterinary medicine , polymerase chain reaction , gene , medicine , zoology , genetics , environmental health
Aims: To feno‐genotypically characterize the Shiga toxin‐producing Escherichia coli (STEC) population in Argentinean dairy cows. Methods and Results: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1 , 52% stx2 and 37% stx1 / stx2. The ehxA , saa and eae were detected in 77%, 66% and 3%, respectively. Thirty‐five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA . One hundred and fifty‐six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2 + , eae + , ehxA + virulence pattern. Conclusions: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. Significance and Impact of the study: The non‐O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.