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Alternatives for biosurfactants and bacteriocins extraction from Lactococcus lactis cultures produced under different pH conditions
Author(s) -
Rodríguez N.,
Salgado J.M.,
Cortés S.,
Domínguez J.M.
Publication year - 2010
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2010.02882.x
Subject(s) - nisin , bacteriocin , lactococcus lactis , lactococcus , microbiology and biotechnology , streptococcaceae , bacteria , biology , extraction (chemistry) , probiotic , food science , chemistry , antimicrobial , lactic acid , chromatography , antibiotics , genetics
Aims: Study of the potential of Lactococcus lactis CECT‐4434 as a biosurfactants and nisin (the only bacteriocin allowed to be used in the food industry) producer for industrial applications, exploiting the possibility of recovering separately both metabolites, taking into account that L. lactis is an interesting micro‐organism with several applications in the food industry because it is recognized as GRAS. Methods and Results: The results showed the ability of this strain to produce cell‐bound biosurfactants, under controlled pH, and cell‐bound biosurfactants and bacteriocins, when pH was not controlled. Three extraction procedures were designed to separately recover these substances. Conclusions: The strain L. lactis CECT‐4434 showed to be a cell‐bound biosurfactants and bacterocins producer when fermentations were carried out under uncontrolled pH. Both products can be recovered separately. Significance and Impact of the Study: Development of a convenient tool for the extraction of cell‐bound biosurfactants and bacteriocins from the fermentation broth.