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Optimization of selected cultivation parameters for Cordyceps guangdongensis
Author(s) -
Lin Q.Y.,
Song B.,
Huang H.,
Li T.H.
Publication year - 2010
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2010.02881.x
Subject(s) - sucrose , cordyceps , potassium nitrate , nitrogen , agar , food science , inoculation , yield (engineering) , horticulture , nitrate , potassium , botany , chemistry , biology , bacteria , materials science , organic chemistry , metallurgy , genetics
Aims: To increase the fruit body production of Cordyceps guangdongensis , selected cultivation conditions, especially nutritional parameters were optimized. Methods and Results: Cordyceps guangdongensis was inoculated on potato dextrose agar slants with pH values from 4·5 to 9·0 and cultivated in artificial media with different carbon and nitrogen supplements. Primordium formation in C. guangdongensis was favoured by slightly acidic conditions. Fruit body yields and biological efficiency (BE) recorded were all highest in cultures of C. guangdongensis supplemented with sucrose and KNO 3 as carbon and nitrogen supplements, respectively. Highest fruit body yields and BE values were recorded with C : N ratio of 12 : 1. The optimal medium consisted of (g l −1 ) 20·0 sucrose, 4·0 soya bean powder, 5·0 beef extract and 10·0 KNO 3 . Cultivation experiments using this medium confirmed its reliability; 18·35% of BE was obtained, compared with a calculated maximum BE of 18·65% based on orthogonal test data. Conclusions: Cordyceps guangdongensis preferred sucrose and potassium nitrate as best carbon and nitrogen supplements. It produced satisfying yield of fruit body with optimized medium. Significance and Impact of the Study: Optimized artificial cultivation conditions could promote the yield of C. guangdongensis and decreased the cost of production.