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Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers
Author(s) -
Halket G.,
Dinsdale A.E.,
Logan N.A.
Publication year - 2010
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02765.x
Subject(s) - microbiology and biotechnology , biology , 16s ribosomal rna , bacillus (shape) , bacteria , kanamycin , antibiotics , genetics
Aims: To evaluate the performance of the VITEK2 Bacillus identification card (BCL) for the identification of aerobic endospore‐forming bacteria, using fresh isolates and reference strains. Methods and Results: One hundred and nine industrial, environmental and clinical isolates were tested using the BCL card. The card contained 46 substrates for measuring carbon source utilization, enzymatic activities, inhibition by 6·5% NaCl and resistance to the antibiotics kanamycin, oleandomycin and polymyxin B. Identifications were made after 14 h incubation, using a database allowing identification of 42 species of the genera Aneurinibacillus , Bacillus , Brevibacillus , Geobacillus , Paenibacillus and Virgibacillus . The reference identities of all isolates were authenticated by phenotypic methods, with 16S rRNA gene sequencing used to resolve discrepancies. Conclusions: One hundred and one strains (93%) were identified correctly to species level, seven strains (6%) were incorrectly identified, and one strain (1%) remained unidentified. Significance and Impact of the Study: The VITEK2 BCL card provides a major advance in the reliable identification of Bacillus species and members of related genera.