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Effects of chlorophyllin on replication of poliovirus and bovine herpesvirus in vitro
Author(s) -
Benati F.J.,
Lauretti F.,
Faccin L.C.,
Nodari B.,
Ferri D.V.,
Mantovani M.S.,
Linhares R.E.C.,
Nozawa C.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02744.x
Subject(s) - poliovirus , chlorophyllin , viral replication , virus , virology , in vitro , biology , cell culture , virus quantification , infectivity , microbiology and biotechnology , chlorophyll , biochemistry , botany , genetics
Abstract Aims: Chlorophyllin (CHLN), a synthetic derivative of chlorophyll, was assayed in the replication of poliovirus (PV‐1) and bovine herpesvirus (BoHV‐1) in HEp‐2 cell cultures. Methods and Results: Virucidal activity of CHLN was evaluated and the time‐of‐addition assay was performed as follows: before the infection (−1 and −2 h), at the time of the infection (0 h) and after the infection (1 and 2 h). Plaque reduction assay (PRA) showed that CHLN inhibited BoHV‐1 and PV‐1 infection and the 50% inhibitory concentrations (IC 50 ) against BoHV‐1 and PV‐1 infection were 8·6 and 19·8 μg ml −1 , respectively. The time‐of‐addition study demonstrated that the CHLN was effective inhibiting viral replication in 51% and 66·5% for PV‐1 and BoHV‐1, respectively, at the highest concentration of 20·0 μg ml −1 , when added during the infection. The directed effect of CHLN on viral strains demonstrated an inhibition of 62% and 66·4% for PV‐1 and BoHV‐1, respectively, by PRA. Conclusions: These results demonstrated that CHLN could be used as an antiviral suggesting directed activity on virus particles and on virus‐receptor sites to BoHV. For poliovirus, CHLN also demonstrated virucide activity, moreover, showed to inhibit early steps of the replication cycle. Significance and Impact of the Study: CHLN demonstrated promising selectivity index for both virus strains; therefore, it can be used for the development of an antiviral agent.