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Production of tylosin in solid‐state fermentation by Streptomyces fradiae NRRL‐2702 and its gamma‐irradiated mutant (γ‐1)
Author(s) -
Khaliq Shazia,
Rashid Nosheen,
Akhtar Kalsoom,
Ghauri Muhammad Afzal
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02720.x
Subject(s) - streptomyces fradiae , tylosin , fermentation , mutant , chemistry , gamma irradiation , microbiology and biotechnology , biology , streptomyces , antibiotics , irradiation , food science , actinomycetales , biochemistry , bacteria , physics , genetics , gene , nuclear physics
Aims: To develop solid‐state fermentation system (SSF) for hyper production of tylosin from a mutant γ‐1 of Streptomyces fradiae NRRL‐2702 and its parent strain. Methods and Results: Various agro‐industrial wastes were screened to study their effect on tylosin production in SSF. Wheat bran as solid substrate gave the highest production of 2500 μg of tylosin g −1 substrate by mutant γ‐1 against parent strain (300 μg tylosin g −1 substrate). The tylosin yield was further improved to 4500 μg g −1 substrate [70% moisture, 10% inoculum (v/w), pH 9·2, 30°C, supplemental lactose and sodium glutamate on day 9]. Wild‐type strain displayed less production of tylosin (655 μg of tylosin g −1 substrate) in SSF even after optimization of process parameters. Conclusion: The study has shown that solid‐state fermentation system significantly enhanced the tylosin yield by mutant γ‐1. Significance and Impact of the Study: This study proved to be very useful and resulted in 6·87 ± 0·30‐fold increase in tylosin yield by this mutant when compared to that of wild‐type strain.