Premium
Comparison of supplements * to enhance recovery of heat‐injured Salmonella from egg albumen
Author(s) -
Gurtler J.B.,
Kornacki J.L.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02695.x
Subject(s) - sodium pyruvate , salmonella , agar , food science , propyl gallate , ferrous , sodium , chemistry , microbiology and biotechnology , biology , biochemistry , bacteria , genetics , organic chemistry , antioxidant
Aims: The purpose of this study was to determine the proficiency of supplements to enhance the recovery of Salmonella from heat‐treated liquid egg albumen on solid agar media. Methods and Results: Salmonella‐ inoculated albumen, heated at 53·3°C for 4 min, was plated on 39 combinations of solid media with or without the addition of 12 supplements. Greater numbers of Salmonella ( P < 0·05) recovered with the addition of 1·0 g l −1 ferrous sulfate (FeSO 4 ) than with any other supplements, except for 0·5 or 1·0 g l −1 3′3′‐thiodipropionic acid (TDP), which recovered equivalent populations. Addition of 1·0 g l −1 sodium pyruvate or 6·0 g l −1 yeast extract plus 1·0 g l −1 sodium pyruvate supported greater resuscitation than unsupplemented tryptic soy agar (TSA) or supplementing with 0·01 or 0·1 g l −1 N‐propyl gallate, 10 g l −1 activated charcoal, 0·1 g l −1 KMnO 4 or 50 mg l −1 ethoxyquin. The remaining supplements supported recovery of equivalent numbers of Salmonella, which were fewer cells than recovered with 1·0 g l −1 FeSO 4 , yet greater populations than recovered with 50 mg l −1 ethoxyquin. Conclusion: Supplementation of plating media with FeSO 4 , TDP or sodium pyruvate enhanced recovery of sublethally injured Salmonella from albumen. Significance and Impact of the Study: Pasteurizing albumen impedes recovery of pathogens. These results suggest that the addition of supplements to plating media may assist resuscitation and colony development of heat‐injured salmonellae.