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Studies on the production of actinomycin‐D by Streptomyces griseoruber – a novel source
Author(s) -
Praveen V.,
Tripathi C.K.M.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02689.x
Subject(s) - streptomyces , biology , azadirachta , bacteria , strain (injury) , metabolite , microbiology and biotechnology , secondary metabolite , organism , fermentation , 16s ribosomal rna , streptomycetaceae , in vitro , actinobacteria , actinomycetales , food science , biochemistry , botany , gene , genetics , anatomy
Aims: To isolate and characterize bioactive metabolites produced by a micro‐organism isolated from a soil sample associated with the roots of a medicinal plant, Azadirachta indica . Methods and Results: Morphological, cultural, physiological and 16S rRNA homology studies revealed that the organism showed 99% similarity with Streptomyces griseoruber NBRC 12873. One bioactive metabolite (Py2) isolated from the fermented broth was characterized as actinomycin‐D (act‐D). It showed high activity against various Gram‐positive and Gram‐negative bacterial cultures, Mycobacterium tuberculosis H37Rv and human neoplastic cells in vitro using standard protocols. Conclusions: The isolated strain S. griseoruber produced act‐D predominantly (210 mg l −1 , c. 88% of the crude) under nonoptimized growth conditions. Significance and Impact of the Study: Streptomyces griseoruber may be exploited as a potential source for the commercial production of act‐D, as this strain is not reported to produce act‐D. Further investigations on the strain for commercial application will be of immense pharmaceutical importance.