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Scanning electron microscope imaging of bacteria based on DNA sequence
Author(s) -
Kenzaka T.,
Ishidoshiro A.,
Tani K.,
Nasu M.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02680.x
Subject(s) - pharmacy , library science , graduate students , medicine , medical education , family medicine , computer science
Aims: To develop a scanning electron microscopic approach using in situ hybridization (SEM–ISH) for gaining both genetic and morphological information about target bacteria. Methods and Results: Target cells were hybridized with DNA‐targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM–ISH enabled to detect low copy number target DNA sequences in individual cells. Conclusions: SEM–ISH allowed the in situ detection of bacteria carrying a specific gene. Significance and Impact of the Study: Combining morphological study with SEM and ISH techniques appears to be a valuable tool to understand the spatial distribution of target cells in complex microbial communities on various materials.