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A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons
Author(s) -
Zhao B.,
Wang H.,
Mao X.,
Li R.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02668.x
Subject(s) - bacteria , chemistry , chromatography , ether , agarose , environmental chemistry , filter paper , organic chemistry , biology , genetics
Aims: A rapid procedure was developed to screen for bacteria that are able to grow on polycyclic aromatic hydrocarbons (PAHs). Methods and Results: A drop of ethyl ether‐dissolved PAH is spread on a sterilized cellulose acetate/nitrate filter lying on the top of a mineral salts agarose plate. After the evaporation of ethyl ether, a serially diluted sample is spread over the filter and incubated. Subsequently, the PAH degrading bacteria can be counted and isolated. Conclusions: This procedure is a simple method for screening bacterial isolates for the ability to grow with PAHs. Significance and Impact of the Study: This technique is rapid to screen and/or count PAH‐degrading bacteria and is also used to streak cultivation without disrupting the PAH layer on plate.