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Isolation and characterization of actinomycetes from healthy goat faeces
Author(s) -
Tan H.,
Deng Z.,
Cao L.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02649.x
Subject(s) - feces , isolation (microbiology) , biology , microbiology and biotechnology , actinomycetaceae , bacteria , actinomycetales , streptomyces , genetics
Aims: To isolate and characterize actinomycetes with probiotic activities from healthy goat faeces. Methods and Results: Faecal actinomycetes were isolated by dilution methods and identified by 16S rRNA gene sequence analysis. The hydrolytic enzyme activities were analysed by clear zone formation. The antimicrobial activities and resistance to heavy metals were tested by growth inhibition methods. The isolates belong to a small group of actinobacterial genera, including Streptomyces , Nocardiopsis and Oerskovia . The Oerskovia was the most widely distributed genus among the cultures. The proportion of streptomycete‐like strains producing amylase or protease is significantly higher than those of other actinomycetes ( P < 0·05). Compared with streptomycete‐like strains, a higher proportion of (α‐ or β‐) galactase‐producing other actinomycetes was found in goat faeces. More than 50% of streptomycete‐like strains showed activities against test fungi. Streptomycetes could tolerate 0·25 mmol l −1 Cr 2 O 7 2− , 2 mmol l −1 Ni 2+ ; however, other actinomycetes are liable to 40 mmol l −1 Fe 3+ and 0·25 mmol l −1 Cr 2 O 7 2− and resistant to 5 mmol l −1 Ni 2+ and 2 mmol l −1 Cu 2+ . Conclusions: The different physiological characteristics of the actinomycetes suggested that the cooperation in the actinomycetes might be involved in their association with goat. Significance and Impact of the Study: Probiotic mixtures based on faecal actinomycetes showed potentials in animal production.