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Decreased time for detection and quantification of virulent Bacillus anthracis and Yersinia pestis using a BioNanoPore (BNP TM ) membrane technology
Author(s) -
Rogers J.V.,
Choi Y.W.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2009.02604.x
Subject(s) - yersinia pestis , bacillus anthracis , library science , clinical microbiology , virulence , microbiology and biotechnology , biology , computer science , bacteria , biochemistry , genetics , gene
Many aspects of biodefense research require quantitative growth assessments of the test agent. This study evaluated the BioNanoPore (BNP™) technology to quantitate Bacillus anthracis and Yersinia pestis faster than traditional plate counting methods. The BNP™ technology enabled quantification of B. anthracis and Y. pestis in phosphate‐buffered saline and naïve rabbit blood at 6 and 24 h, respectively. After 6 h of growth, counts for B. anthracis ranged from 6·19–6·45 log 10 CFU ml −1 on BNP™, while counts after 24 h on tryptic soy agar (TSA) ranged from 6·51–6·58 log 10 CFU ml −1 . For Y. pestis , counts on BNP™ at 24 h ranged from 6·31–6·41 log 10 CFU ml −1 on BNP™ and ranged from 6·44–6·89 log 10 CFU ml −1 on TSA at 48 h. This study demonstrates that the BNP™ technology provides a more rapid detection of B. anthracis and Y. pestis , which could aid in the evaluation of potential medical countermeasures and treatments as well as other biological defense applications such as surface sampling or decontamination efficacy.