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Development of a defined medium for Clostridium scatologenes ATCC 25775
Author(s) -
Kridelbaugh D.,
Doerner K.C.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02546.x
Subject(s) - clostridium , microbiology and biotechnology , bacteria , clostridiaceae , biology , food science , genetics , toxin
Aims: To develop a defined medium for Clostridium scatologenes ATCC 25775, which produces the malodorants 3‐methylindole (skatole) and 4‐methylphenol ( p‐ cresol). Methods and Results: Clostridium scatologenes was cultured in anaerobic broth medium (pH 6·3) at 37°C containing ammonia, minerals and a commercial vitamin solution. Data indicate α‐ketoglutarate, l‐ glutamate or l‐ glutamine is a required nutrient that can also serve as a primary carbon and energy source. When cultured in defined medium containing glutamate; glucose, fructose and betaine served as primary carbon and energy sources. l‐ Tryptophan, l‐ tyrosine, sorbitol and indole acetic acid did not enhance growth. In the absence of tryptophan, cells produced indole when grown using glucose or fructose. 4‐Methylphenol was produced when growing cells were supplied with tyrosine. When supplied with tryptophan, 3‐methylindole was produced by glucose‐ or fructose‐growing cells but not from glutamate‐growing cells. Cells grown in the presence of pyruvate produced indole, 3‐methylindole and 4‐methylphenol. Conclusions: Clostridium scatologenes requires α‐ketoglutarate, l‐ glutamate, or l‐ glutamine for growth in defined medium. Cells produce indole when glucose or fructose is included in defined medium. Significance and Impact of the Study: The development of a defined medium will assist in physiology studies and genetic analysis of this strain.