Premium
Bioluminescence intensity difference observed in luminous bacteria groups with different motility
Author(s) -
Sasaki S.,
Okamoto T.,
Fujii T.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02525.x
Subject(s) - bioluminescence , photobacterium , bacteria , luminescence , motility , intensity (physics) , biology , light intensity , luminescent bacteria , chemistry , biophysics , microbiology and biotechnology , vibrio , biochemistry , materials science , optics , physics , optoelectronics , genetics
Aims: The aim of this study was to compare the luminescent intensity of bioluminescence from marine luminous bacteria with different motility. Methods and Results: Luminescent bacteria were separated according to their motility using a microfluidic device. The cell densities of the separated samples were measured using a counting plate. The luminescent intensity of the separated samples was measured using a luminometer. The luminescent intensity per cell was calculated, and the values from the mobile (swimmers) and the nonmobile cells (nonswimmers) per single cell were compared; as a result, the former were proved to be larger than the latter. Conclusions: Microfluidics were shown to be effective for the separation of bioluminescent bacteria and the bioluminescent intensity difference per cell was recognized with this experiment. Significance and Impact of the Study: This study introduced for the first time a method to examine the individual cell function of Photobacterium kishitanii .