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Comparison of T‐RFLP and DGGE techniques to assess denitrifier community composition in soil
Author(s) -
Enwall K.,
Hallin S.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02498.x
Subject(s) - restriction fragment length polymorphism , terminal restriction fragment length polymorphism , biology , nitrous oxide reductase , temperature gradient gel electrophoresis , genetics , bacteria , gene , polymerase chain reaction , ecology , nitrite reductase , nitrate , nitrate reductase , 16s ribosomal rna
Terminal restriction fragment length polymorphism (T‐RFLP) and denaturing gradient gel electrophoresis (DGGE) and subsequent statistical analysis were compared with assess denitrifier community composition in agricultural soil based on the nosZ gene, encoding the nitrous oxide reductase. Analysis of binary or relative abundance‐based metric and semi‐metric distance matrices provided similar results for DGGE, but not for T‐RFLP. Moreover, DGGE had a higher resolution than T‐RFLP and binary data was better for discriminating between samples.