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Evaluation of a digoxigenin‐labelled probe for detection of Aeromonas spp.
Author(s) -
Khushiramani R.,
Girisha S.K.,
Karunasagar I.,
Karunasagar I.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02491.x
Subject(s) - aeromonas , digoxigenin , biology , microbiology and biotechnology , chromatography , bacteria , chemistry , genetics , gene , in situ hybridization , gene expression
An outer membrane protein‐based Digoxigenin (DIG)‐labelled DNA probe was developed for the specific detection of Aeromonas sp. from food/environmental/clinical samples. Dot blot reaction answered for all the Aeromonas isolates and was negative for Escherichia coli , Pseudomonas sp., Klebsiella sp., Vibrio parahaemolyticus , V. harveyi , V. alginolyticus , V. vulnificus . Edwardsiella tarda and Staphylococcus sp. As this protein is highly conserved in various Aeromonas species, the probe has the potential for use as a rapid and reliable diagnostic tool.