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Isolation and characterization of alginate‐degrading bacteria for disposal of seaweed wastes
Author(s) -
Tang J.C.,
Taniguchi H.,
Chu H.,
Zhou Q.,
Nagata S.
Publication year - 2009
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02481.x
Subject(s) - isolation (microbiology) , algae , bacteria , biodegradation , microbiology and biotechnology , pulp and paper industry , biology , chemistry , botany , ecology , engineering , genetics
Aims: Isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process. Methods and Results: Decomposition of alginate polymers was checked by the 3,5‐dinitrosalicylic acid (DNS) method for reducing sugar, and absorbance at 235 nm for unsaturated sugar. A bacterium A7 was isolated from wakame compost and confirmed to belong to the genus Gracilibacillus by partial 16S rDNA analysis. The optimum condition for the growth of A7 in a medium containing 5 g l −1 of sodium alginate is as follows: pH, 8·5–9·5; NaCl, 0·5 mol l −1 ; temperature, 30°C and polypeptone as nutrient content, 2–5 g l −1 . In a laboratory‐scale composting experiment, the alginate content in wakame compost decreased to 14·3% after 72 h of composting from an initial value of 36%, indicating the effectiveness of alginate decomposition of A7 in wakame composting. Conclusions: The bacterium A7 was found to be alginate lyase‐producing in genus Gracilibacillus and effective in degrading alginate to oligosaccharides in wakame during composting process. Significance and Impact of the Study: Development of new methods for the disposal of marine wastes and production of functional products.