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Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest
Author(s) -
Li J.,
Zhao G.Z.,
Chen H.H.,
Wang H.B.,
Qin S.,
Zhu W.Y.,
Xu L.H.,
Jiang C.L.,
Li W.J.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02470.x
Subject(s) - antimicrobial , biology , microbiology and biotechnology , polyketide , candida albicans , plant use of endophytic fungi in defense , staphylococcus aureus , klebsiella pneumoniae , staphylococcus epidermidis , streptomyces , escherichia coli , bacteria , botany , biochemistry , gene , biosynthesis , genetics
Aims: The aim of this study was to screen antitumour and antimicrobial activities of endophytic actinomycetes isolated from pharmaceutical plants in rainforest in Yunnan province, China. Methods and Results: Antitumour activity was studied by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay and antimicrobial activity was determined by agar well diffusion method. The high bioactive endophytic isolates were identified and further investigated for the presence of polyketide synthases (PKS‐I, PKS‐II) and nonribosomal peptide synthetases (NRPS) sequences by specific amplification. The molecular identification confirmed that the 41 isolates showed significant activities were members of the genus Streptomyces . Among them, 31·7% of endophytic streptomycete cultures were cytotoxic against A549 cells, 29·3% against HL‐60 cells, 85·4% against BEL‐7404 cells, 90·2% against P388D1 cells, 65·9% were active against Escherichia coli , 24·4% against Staphylococcus aureus , 31·7% against Staphylococcus epidermidis , 12·2% against Candida albicans and no strain displayed antagonistic activity against Klebsiella pneumoniae . High frequencies of positive PCR amplification were obtained for PKS‐I (34·1%), PKS‐II (63·4%) and NRPS (61·0%) biosynthetic systems. Conclusions: Many endophytic streptomycetes isolated from pharmaceutical plants in rainforest possess remarkable and diverse antitumour and antimicrobial bioactivities. Significance and Impact of the Study: These endophytic streptomycetes are precious resources obtained from rainforests, and they could be a promising source for bioactive agents.