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Influence of baking enzymes on antimicrobial activity of five bacteriocin‐like inhibitory substances produced by lactic acid bacteria isolated from Lithuanian sourdoughs
Author(s) -
Narbutaite V.,
Fernandez A.,
Horn N.,
Juodeikiene G.,
Narbad A.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02466.x
Subject(s) - bacteriocin , antimicrobial , bacteria , lactic acid , lithuanian , microbiology and biotechnology , enzyme , chemistry , biology , biochemistry , linguistics , philosophy , genetics
Aim: To evaluate the effect of four different baking enzymes on the inhibitory activity of five bacteriocin‐like inhibitory substances (BLIS) produced by lactic acid bacteria (LAB) isolated from Lithuanian sourdoughs. Methods and Results: The overlay assay and the Bioscreen methods revealed that the five BLIS exhibited an inhibitory effect against spore germination and vegetative outgrowth of Bacillus subtilis , the predominant species causing ropiness in bread. The possibility that the observed antibacterial activity of BLIS might be lost after treatment with enzymes used for baking purposes was also examined. Conclusions: The enzymes tested; hemicellulase, lipase, amyloglucosidase and amylase had little or no effect on the majority of the antimicrobial activities associated with the five BLIS studied. Significance and Impact of the Study: This study suggests a potential application in the sourdough baking industry for these antimicrobial producing LAB strains in the control of B. subtilis spore germination and vegetative outgrowth.