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The detection of possible sensor histidine kinases regulating citrate/malate metabolism from the bovine rumen microbial ecosystem
Author(s) -
Sun W.,
Mitsumori M.,
Takenaka A.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02460.x
Subject(s) - rumen , metabolism , kinase , biochemistry , biology , histidine , histidine kinase , bacteria , chemistry , microbiology and biotechnology , enzyme , genetics , fermentation
Aims: To detect sensor histidine protein kinases (HPKs) similar to accessory gene regulator C (AgrC) from the rumen microbial ecosystem. Methods and the Results: Genes related to sensor HPKs were amplified by PCR using two pairs of agr C‐specfic primers from DNA extracted from bovine rumen contents. The PCR products were cloned, sequenced and phylogenetically analysed. It appeared that two sequences were HPKs. Conclusions: Although amino acid sequences deduced from the nucleotide sequences obtained in this study showed high similarities with sensor HPKs responding to citrate or C 4 ‐dicarboxylates, they did not show high similarities with AgrC. Significance and Impact of the Study: This study revealed the presence in the rumen of sensor HPKs responding to citrate or C 4 ‐dicarboxylates, which could stimulate rumen fermentation. Therefore, it has been shown that citrate or C 4 ‐dicarboxylate metabolism is partially regulated by a two‐component regulatory system in some rumen bacteria.