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Molecular characterization of early colonizer bacteria from wastes in a steel plant
Author(s) -
Freitas D.B.,
LimaBittencourt C.I.,
Reis M.P.,
Costa P.S.,
Assis P.S.,
ChartoneSouza E.,
Nascimento A.M.A.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02415.x
Subject(s) - bacteria , micrococcus luteus , bacillus cereus , biology , micrococcus , microbiology and biotechnology , 16s ribosomal rna , acinetobacter , pseudomonas , bacillus (shape) , staphylococcus aureus , genetics
Aims: Forty‐nine bacteria isolated from four newly‐produced waste samples of a steel industry, which had a high content of CaO, MgO, Cr and P 2 O 5 , were characterized molecularly and phenotypically by susceptibility testing against heavy metals. Methods and Results: Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to nine genera, Pseudomonas , Micrococcus , Acinetobacter , Bacillus , Dietzia , Kocuria , Diaphorobacter , Staphylococcus and Brevibacillus . Besides, some isolates could be affiliated to species: M. luteus , Ac. junii , Ac. schindleri , B. cereus , K. marina , D. nitroreducens and Staph. warneri. The bacteria that were characterized are taxonomically diverse, and Pseudomonas and Micrococcus predominated. Fingerprinting BOX‐PCR revealed high genomic heterogeneity among the isolates. Among the heavy metal compounds Zn, Ni, Pb and Cu were least toxic to the bacterial isolates, whereas Ag inhibited all isolates at 0·001 mmol l −1 . Conclusions: Heterotrophic bacteria, affiliated with several phylogentic groups, were able to colonize different wastes of a steel industry. Significance and Impact of the Study: This study extends our knowledge of the early colonizers bacteria populating siderurgic environments. Some of these bacteria could have potential for recycling siderurgic waste for steel production.