Premium
Characterization of probiotic carnobacteria isolated from rainbow trout ( Oncorhynchus mykiss ) intestine
Author(s) -
Kim D.H.,
Austin B.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02401.x
Subject(s) - vibrio anguillarum , microbiology and biotechnology , streptococcus iniae , biology , rainbow trout , probiotic , aeromonas salmonicida , bacteria , aeromonas hydrophila , vibrio , fish <actinopterygii> , fishery , genetics
Aims: To characterize two probiotic carnobacterial isolates, Carnobacterium maltaromaticum (B26) and C. divergens (B33), derived from rainbow trout ( Oncorhynchus mykiss ) intestine. Methods and Results: Both cultures, which were able to colonize the fish gut mucosal layer, comprised nonsporogenous, nonmotile, Gram‐positive, catalase and oxidase‐negative rods. The growth of both carnobacteria occurred between 0 and 37°C, in 0–10% (w/v) NaCl and at pH 5–10. Specifically, strain B26 grew in nutrient broth supplemented with 15% (w/v) NaCl. The most abundant cellular fatty acid of both cultures was 9‐octadecenoic acid (18 : 1 n ‐9) (B26 = 52·6%; B33 = 40·6%), which was characteristic of Carnobacterium . Both cultures were inhibitory to Aeromonas salmonicida , Aer. hydrophila , Streptococcus iniae and Vibrio anguillarum , and strain B33 inhibited Listeria monocytogenes . Both carnobacteria, which did not contain plasmids, produced inhibitory compounds against Gram‐positive and Gram‐negative bacteria. Conclusions: Both probiotic cultures, B26 and B33, had unique phenotypic characteristics and showed a broad spectrum of antibiotic resistance against varying pathogenic bacteria. Significance and Impact of the Study: The results of this study contribute to new information and significance of carnobacterial species.