Premium
Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials
Author(s) -
Sharma M.,
Chadha B.S.,
Kaur M.,
Ghatora S.K.,
Saini H.S.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02357.x
Subject(s) - thermophile , internal transcribed spacer , xylanase , biology , phylogenetic tree , ribosomal dna , microbiology and biotechnology , genetics , gene , bacteria , enzyme , biochemistry
Aims: Molecular characterization of commercially important group of xylanase producing thermophilic/thermotolerant fungi. Methods and Results: DNA from 16 thermophilic/thermotolerant fungal isolates was amplified by PCR using three sets of primers: (i) internal transcribed spacer sequence (ITSI‐5·8S‐ITSII), (ii) D1/D2 hyper variable region of 26S rDNA and (iii) 18S rDNA region. The amplified products of internal transcribed spacers (ITS) and D1/D2 region were sequenced and analysed using C lustal X, whereas, amplified 18S rDNA region was subjected to RFLP analysis based on restriction digestion with Rsa I, Mbo I and Hinf I. Conclusions: The sequence based analyses of ITSI‐5·8S‐ITSII as compared with D1/D2 region of 26–28S rDNA was found to be a better tool for phylogenetic resolution of thermophilic/thermotolerant fungi. The ITSI‐5·8S‐ITSII sequence‐based dendrogram indicates an early divergence of the alkaline active xylanase producing thermophilic fungal strains. Significance and Impact of the Study: This study was the first report on phylogenetic characterization of thermophilic/thermotolerant fungi.