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Fatty acid analysis as a chemotaxonomic tool for taxonomic and epidemiological characterization of four fish pathogenic Tenacibaculum species
Author(s) -
PiñeiroVidal M.,
Pazos F.,
Santos Y.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02348.x
Subject(s) - fish <actinopterygii> , biology , humanities , zoology , art , fishery
Aims: In this work, fatty acid content and profiles were analysed in order to differentiate the species Tenacibaculum maritimum , Tenacibaculum gallaicum , Tenacibaculum discolor and Tenacibaculum ovolyticum that are pathogenic for cultured marine fish and to assess the potential of fatty acid profiles as a tool for epizootiological typing. Methods and Results: The fatty acid methylesters (FAMEs) were extracted from cells grown on marine agar for 48 h at 25°C and were prepared and analysed according to the standard protocol of the MIDI/Hewlett Packard Microbial Identification System. The cellular fatty acid profiles of Tenacibaculum strains tested were characterized by the presence of large amounts of branched (36·1–40·2%) and hydroxylated (29·6–31·7%) fatty acids. The FAME products from the four species significantly ( P < 0·05) differed in the content of iso‐C 15:0 3‐OH, iso‐C 16:0 3‐OH, iso‐C 15:1 G, summed feature 3 (a component that contains C 16:1 ω7c and/or iso‐C 15:0 2‐OH), iso‐C 16:0 , C 17:1 ω6c, C 15:0 3‐OH, iso‐C 17:0 3‐OH. Conclusions: Results of present study demonstrated the existence of differences in the fatty acids content between the T. maritimum isolates from different marine fish/geographical origin and between strains of T. maritimum , T. discolor , T. gallaicum and T. ovolyticum. Significance and Impact of the Study: Profiling of fatty acids may be a useful tool to distinguish T. maritimum from other Tenacibaculum species pathogenic for fish as well as for epizootiological differentiation of T. maritimum isolates.