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Early molecular diagnosis and detection of Puccinia striiformis f. sp. tritici in China
Author(s) -
Lihua C.,
Shichang X.,
Ruiming L.,
Taiguo L.,
Wanquan C.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02313.x
Subject(s) - puccinia striiformis , primer (cosmetics) , biology , stripe rust , polymerase chain reaction , basidiomycota , genomic dna , gene , puccinia , dna sequencing , genetics , dna , microbiology and biotechnology , botany , plant disease resistance , chemistry , mildew , organic chemistry
Aims: Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici ( Pst ) , is the most important foliar disease on wheat in China. Early molecular diagnosis and detection of stripe rust will provide a useful aid to the accurate forecast and seasonal control of this destructive disease. Our objective was to develop PCR assays for the rapid identification and detection of P. striiformis . Methods and Results: The genomic DNA of P. striiformis and P. triticina were amplified by a pair of primers derived from conserved β‐tubulin gene sequence. A 235‐bp specific DNA fragment of P. striiformis was isolated and purified. Based on its sequence, another two primer sets were designed successfully to obtain new sequence‐characterized amplified region (SCAR) markers of P. striiformis , which could be amplified in all test isolates of P. striiformis , whereas no DNA fragment was obtained in other nontarget wheat pathogens. The detection limit of the primer set YR (f)/YR (r1) was 2·20 pg μl −1 . The new SCAR markers of P. striiformis can also be detected in Pst ‐infected wheat leaves postinoculated for 2 days. Conclusions: Our assays are significantly faster than the conventional methods used in the identification of P. striiformis . Significance and Impact of the Study: Development of a simple, high‐throughput assay kit for the rapid diagnosis and detection of wheat stripe rust would be anticipated in a further study.