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Cytidine deamination assay to differentiate Staphylococcus aureus from other staphylococci
Author(s) -
Krasuski A.,
MichnowskaSwincow E.,
Jarzembowski T.
Publication year - 2007
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02218.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , deamination , coagulase , cytosine , cytidine deaminase , polymerase chain reaction , staphylococcus , gene , bacteria , genetics , enzyme , biochemistry
Aims: Adoption of the property of cytidine (cytosine‐β‐ d ‐riboside) deamination in staphylococci to distinguish Staphylococcus aureus from other staphylococci. Methods and Results: A total of 560 staphylococcal strains were examined. The test demonstrated a sensitivity of 97·1% and a specificity of 98·8%. Of the 249 S . aureus strains (115 oxacillin‐resistant) 58 strains were coagulase‐negative S . aureus and another 16 strains were clumping factor‐negative S . aureus . The 74 deficient S . aureus strains were identified by 16S rRNA gene sequencing and further investigated by spa typing and 13 spa types were found. Conclusions: The cytidine deaminase test (CDT) is useful especially for distinguishing coagulase‐ and clumping factor‐negative S . aureus from other staphylococci and the results correlated well with 16S rRNA sequencing and the polymerase chain reaction (PCR) amplification of the nuc gene. Significance and Impact of the Study: Cytidine deamination assay differentiates S . aureus from other staphylococci. This method is fast (6 h) and reliable in distinguishing between non‐ S . aureus and the defective (coagulase‐negative, clumping factor‐negative) S . aureus isolates which could have major consequences for therapy.