Premium
A novel multiplex PCR for the identification of Vibrio parahaemolyticus , Vibrio cholerae and Vibrio vulnificus
Author(s) -
Bauer A.,
Rørvik L.M.
Publication year - 2007
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02195.x
Subject(s) - vibrio cholerae , vibrio parahaemolyticus , vibrio vulnificus , microbiology and biotechnology , multiplex polymerase chain reaction , vibrio , biology , vibrio alginolyticus , polymerase chain reaction , vibrionaceae , primer (cosmetics) , bacteria , gene , genetics , chemistry , organic chemistry
Aim: To establish a simple multiplex polymerase chain reaction (PCR) that will identify Vibrio parahaemolyticus , Vibrio cholerae and Vibrio vulnificus. Methods and Results: A total of 429 Vibrio spp. from various origins were tested with the novel primers targeting toxR . The reverse primers were all designed to be species specific, while the forward primer was universal. The primers correctly identified all the V. parahaemolyticus , V. cholerae and V. vulnificus isolates tested. Conclusions: The toxR multiplex PCR works well when the initial colony morphology is known. If not, Vibrio alginolyticus might represent a diagnostic obstacle. Significance and Impact of the Study: The method provides a fast and reliable way of identifying the main Vibrio spp. involved in food‐borne disease. The method could prove very useful for laboratories working with identification of these Vibrio spp.