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Evaluation of different growth media for the recovery of the species of Alicyclobacillus
Author(s) -
Witthuhn R.C.,
Duvenage W.,
Gouws P.A.
Publication year - 2007
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02182.x
Subject(s) - agar , food science , orange juice , yeast extract , inoculation , pear , starch , chemistry , agar plate , endospore , orange (colour) , bacteria , biology , spore , microbiology and biotechnology , botany , fermentation , horticulture , genetics
Aims: Five different isolation media, namely potato dextrose agar (PDA), orange serum agar (OSA), K agar, yeast–starch–glucose agar and Bacillus acidocaldarius medium were evaluated for the recovery of Alicyclobacillus spp. from inoculated diluted and undiluted fruit‐juice concentrates. Methods and Results: Plates of PDA (pH 3·7), spread with vegetative cells (3·9 × 10 6 CFU ml −1 ) of Alicyclobacillus acidoterrestris from single‐strength pear juice, recovered 2·9 × 10 6 CFU ml −1 after 5 days at 50°C (74% recovery). The recovery of endospores from single‐strength pear juice, after a heat treatment at 80°C for 10 min, was higher on spread plates of OSA (pH 5·5) at 50°C for 5 days (97% recovery). Conclusions: PDA (pH 3·7) and OSA (pH 5·5) at 50°C for 3–5 days recovered the highest numbers of vegative cells and endospores of Alicyclobacillus spp. from sterilized fruit juices and concentrates. Significance and Impact of the Study: The most appropriate synthetic media for the recovery of Alicyclobacillus species from inoculated fruit juices and concentrates are shown.