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Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology
Author(s) -
Szita G.,
Gyenes M.,
Soós L.,
Rétfalvi T.,
Békési L.,
Csikó G.,
Bernáth S.
Publication year - 2007
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2007.02145.x
Subject(s) - pseudomonas aeruginosa , microbiology and biotechnology , bacteria , pseudomonas , pseudomonadaceae , pseudomonadales , biology , chromatography , chemistry , genetics
Aims: To investigate whether the use of a novel synthetic medium in conjunction with impedimetric technology could provide a rapid and automated detection of Pseudomonas aeruginosa in water samples. Methods and Results: A selective synthetic medium (Z‐broth) in which the only carbon and nitrogen source is acetamide was applied in direct impedimetric examination for the selective isolation of P. aeruginosa. A total of 1036 tap‐water, well‐water, swimming‐pool water and dialysis water samples were investigated, and any P. aeruginosa contamination was detected in 7–24 h. Neither false‐negative nor false‐positive results were observed. Conclusions: The results of the present evaluation demonstrate that impedance measurement with the use of Z‐broth is suitable for the rapid and automatic detection of P. aeruginosa in water . Significance and Impact of the Study: The main advantages of the method: 240 samples can be examined in one step, the procedure is fully automated, the results are obtained quickly and the labour and media requirements are low.