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A study on medium chain length‐polyhydroxyalkanoate accumulation in Escherichia coli harbouring phaC 1 gene of indigenous Pseudomonas sp. LDC‐5
Author(s) -
Sujatha K.,
Shenbagarathai R.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.02016.x
Subject(s) - polyhydroxyalkanoates , escherichia coli , pseudomonas , microbiology and biotechnology , indigenous , gene , bacteria , biology , pseudomonadaceae , pseudomonadales , enterobacteriaceae , chain (unit) , chemistry , genetics , physics , ecology , astronomy
Aims: This study is mainly focused on the heterologous expression and accumulation of polyhydroxyalkanoates (PHA) in Escherichia coli . Methods andResults: PHA synthase gene ( phaC 1) from indigenous Pseudomonas sp. LDC‐5 wasamplified by PCR and cloned in E. coli (Qiagen EZ competent cells). Therecombinant E. coli was analysed and confirmed for its expression of phaC 1 gene by phase contrast microscopy, Western blot analysis andspectral studies (Fourier‐transform infrared spectroscopy). It was furtherevaluated for its accumulation in different carbon and nitrogen sources. Theaccumulation of PHA(3·4 g l −1 ) was enhanced in the medium supplemented with glycerol and fish peptone compared to the other carbon and nitrogen sources used in this study. Conclusions: This study would enable the reduction of cost of PHA production. Significance and Impact ofthe Study: An important part of this study is that E. coli harbouring partial phaC 1 gene could accumulate medium chain length PHA significantly. Theresults demonstrated that the E. coli strain could be a potential candidate for the large‐scale production of polymer. The conditions for the higher yield and productivity will be optimized in the next phase using fermentation studies.