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Inhibitory effects of Lactobacillus acidophilus lysates on the cytotoxic activity of shiga‐like toxin 2 produced from Escherichia coli O157:H7
Author(s) -
Kim Y.,
Han K.S.,
Imm J.Y.,
Oh S.,
You S.,
Park S.,
Kim S.H.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.02005.x
Subject(s) - lactobacillus acidophilus , escherichia coli , shiga toxin , microbiology and biotechnology , biology , toxin , virulence , cytotoxic t cell , cytotoxicity , in vivo , shiga like toxin , bacteria , in vitro , probiotic , biochemistry , genetics , gene
Aims: The purpose of this study was to characterize the degree to which four cell lysates obtained from Lactobacillus acidophilus strains affected the cytotoxic activity of Escherichia coli O157:H7 in vitro and in vivo . Methods and Results: In a cytotoxic inhibition test that used 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and toxin‐binding ELISA assays, the activity of shiga‐like toxin 2 (Stx‐2) was inhibited profoundly by the cell lysates (10 mg ml −1 ) from two strains of L. acidophilus A4 and 30SC (>85% of survival rates compared with the control) among the five strains tested. In particular, a significant decline in the virulence level of E. coli O157:H7, under the presence of the cell lysates of L. acidophilus A4, was observed by killing assay of Caenorhabditis elegans in vivo model. Conclusions: According to our results, L. acidophilus strains might be capable of attenuating the virulence of Stx‐2 produced from E. coli O157:H7. Significance and Impact of the Study: The cell lysates of L. acidophilus can be applied to a variety of foods, and can be used as adjuncts for the inhibition of Stx‐2‐mediated cytotoxicity.