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In vitro growth inhibition of Helicobacter pylori by lactobacilli belonging to the Lactobacillus plantarum group
Author(s) -
Rokka S.,
Pihlanto A.,
Korhonen H.,
Joutsjoki V.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01998.x
Subject(s) - lactobacillus plantarum , helicobacter pylori , microbiology and biotechnology , biology , lactobacillus , in vitro , antibiotics , bacteria , intracellular , growth inhibition , lactobacillaceae , fermentation , lactic acid , biochemistry , genetics
Aims: The aim of this study was to test and locate the in vitro anti‐ Helicobacter activity of seven Lactobacillus strains belonging to Lactobacillus plantarum group. Methods and Results: Growth inhibition of H. pylori was tested using a well‐plate assay. Of the strains displaying the strongest growth inhibition, a L. plantarum isolated from sauerkraut (MLBPL1) was chosen for further studies. The detected anti‐ Helicobacter activity of MLBPL1 was mainly associated with cell wall, and to a minor extent with the culture supernatant. The active component, which was determined to be between 3 and 10 kDa in size, retained its activity after 10 min treatment at 100°C. The activity was present when MLBPL1 was cultivated in rich laboratory cultivation medium MRS and in different food matrices. Conclusions: The strains belonging to L. plantarum group showed anti‐ Helicobacter activity in vitro . The main activity seemed to be associated with cell wall rather than culture supernatant or intracellular fraction. Significance and Impact of the Study: In view of the rapid spread of resistant H. pylori strains caused by antibiotic therapy, addition of a fermented food containing L. plantarum to the conventional antibiotic treatment of Helicobacter infection could establish a potential complementary means to suppress the infection.