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Media‐ and method‐dependent variations in minimal inhibitory concentrations of antiplaque agents on oral bacteria
Author(s) -
Haraszthy V.I.,
Reynolds H.S.,
Sreenivasan P.K.,
Subramanyam R.,
Cummins D.,
Zambon J.J.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01961.x
Subject(s) - antimicrobial , triclosan , agar dilution , microbiology and biotechnology , minimum inhibitory concentration , saliva , bacteria , agar plate , agar , biology , dental plaque , minimum bactericidal concentration , serial dilution , chemistry , medicine , biochemistry , genetics , pathology , alternative medicine
Aims: To determine minimal inhibitory concentrations (MICs) and the percentage of nonsusceptible bacteria – those still cultivable above a threshold concentration – in human supragingival dental plaque and saliva for antiplaque/antimicrobial agents including triclosan (TCS) and trichlorocarbanilide (TCC), and a new potential antimicrobial, 2‐ t ‐butyl‐5‐(4‐ t ‐butylphenyl)‐phenol (DTBBP). Methods and Results: Broth and agar dilution‐based MIC tests were performed using 28 oral and nonoral bacterial strains representing 17 species. MICs for TCS were lowest and more than 100‐fold lower than DTBBP ( P < 0·0005) by both methods. MICs for TCS were lower in broth‐based tests compared with TCC. The additions of defibrinated blood to agar and horse serum to broth increased MICs – in the case of TCS, 10‐ to 15‐fold. Significantly higher proportions of nonsusceptible plaque and salivary bacteria were recovered from agar media containing DTBBP or TCC compared with TCS ( P < 0·05). Conclusions: TCS is a more effective antimicrobial agent than either TCC or DTBBP as determined by in vitro testing. Significance and Impact of the Study: The utility of in vitro testing for antiplaque agents as a predictor of in vivo efficacy is affected by the methods used.