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Application of a microfluidic device for counting of bacteria
Author(s) -
Inatomi K.I.,
Izuo S.I.,
Lee S.S.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01953.x
Subject(s) - microfluidics , nozzle , microscope , syringe driver , materials science , charge coupled device , fluorescence microscope , inverted microscope , spectrum analyzer , microfluidic chip , cell counting , fluorescence , optics , chromatography , biomedical engineering , syringe , nanotechnology , chemistry , physics , cell , engineering , mechanical engineering , biochemistry , cell cycle , thermodynamics
Aims: To develop a miniaturized analytical system for counting of bacteria. Methods and Results: Escherichia coli cells were used throughout the experiments. The system consists of a microfluidic chamber, a fluorescence microscope with a charge‐coupled device (CCD) camera and syringe pumps. The chamber was made of a silicone rubber (30 × 30 mm and 4 mm high). The E. coli cells were flowed from a micro‐nozzle fabricated in the chamber and detected with the CCD camera. The individual cells were indicated as signal peaks on a computer. The cell counts showed a good correlation compared with that of a conventional plate counting method, and results of the simultaneous detection of live and dead cells were also presented. Conclusions, Significance and Impact of the Study: The system having a small disposable nozzle has the advantages for low cost and safe medical or environmental analysis, when compared with a conventional flow cytometer. This is the first step of the development of a one‐chip microbe analyzer.