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Isolation from poultry litter and characterization in high phosphate conditions of Staphylococcus spp. capable of growth
Author(s) -
Vadari Y.,
Mason B.P.,
Doerner K.C.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01901.x
Subject(s) - phosphate , poultry litter , brain heart infusion , biology , litter , microbiology and biotechnology , intracellular , inorganic phosphate , staphylococcus , food science , bacteria , staphylococcus aureus , biochemistry , nutrient , ecology , agar , genetics
Aims: To isolate and characterize micro‐organisms from poultry litter capable of growing under phosphate concentrations typical of poultry litter. Methods and Results: Poultry litter extracts were plated onto brain‐heart infusion medium (BHI) containing an additional 0·75 mol l −1 phosphate (BHI‐P). Colonies were screened for the presence of inclusion granules with five being selected for further study. All strains displayed identical biochemical characteristics consistent with Staphylococcus spp. and grouped with Staphylococcus spp. by comparative 16S rDNA analysis. Thus all five strains were identified as such. All strains displayed elevated intracellular phosphate levels when cultured in BHI‐P broth (0·417–0·600 μ g phosphate mg −1 protein) vs BHI broth (0·075–0·093 μ g phosphate mg −1 protein). When grown using an austere semi‐defined medium or BHI‐P, Staph . sp. #7 displayed similar elevated intracellular phosphate levels compared with growth in BHI. Conclusions: Poultry litter contains novel Staphylococcus spp. capable of robust growth when exposed to phosphate levels comparable with that typically found in poultry litter. Data suggest intracellular phosphate levels in these strains increase in response to increasing phosphate in the medium or austere medium conditions. Intracellular phosphate did not reach levels comparable with known hyper‐accumulating micro‐organisms. Significance and Impact of the Study: These data suggest poultry litter possesses a resident microflora that thrives and accumulates intracellular phosphate in response to high phosphate conditions.