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Effect of the cryopreservation conditions on the viability of the rumen ciliate Diploplastron ( Metadinium ) affine
Author(s) -
De La Fuente G.,
Cebrián J.A.,
Fondevila M.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01894.x
Subject(s) - cryopreservation , library science , humanities , biology , philosophy , computer science , microbiology and biotechnology , embryo
Aims: To study the viability of Diploplastron ( Metadinium ) affine after its cryopreservation at two cooling rates, and the effect of procedure conditions on viability. Methods and Results: There were differences in viability between cooling rates (1 and 4°C min −1 ) at 15 or 5°C, but not after thawing. When the equilibrium temperature (25 or 5°C), the cryopreservant (glycerol or dimethyl sulfoxide [DMSO]) and the use of membrane protector were tested, there were no differences caused by the cryopreservant or the membrane protector. However, the equilibrium at 25°C increased the viability ( P = 0·005) compared with 5°C. Conclusions: Viability after thawing was 0·10–0·19. Adding the cryopreservant (either glycerol or DMSO) at 25°C instead of 5°C improves viability of D. affine after thawing. Significance and Impact of the Study: Conditions of cryopreservation are largely dependent on the species of rumen protozoa. Number of viable cells after thawing would indicate the possibility of culture recovery for D. affine .