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Production of exopolysaccharides by Agrobacterium sp. CFR‐24 using coconut water – a byproduct of food industry
Author(s) -
Shivakumar S.,
Vijayendra S.V.N.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01881.x
Subject(s) - fermentation , food industry , food science , sucrose , pulp and paper industry , microbiology and biotechnology , biology , chemistry , engineering
Aims: The work is intended to explore the suitability of underutilized coconut water (a byproduct of food industry) for the production of exopolysaccharides (EPS) by Agrobacterium sp. CFR 24. Methods and Results: Besides checking the suitability of coconut water for the production of water‐soluble (WS) and water‐insoluble (WIS) EPS, certain fermentation parameters, such as initial pH, incubation period and kinetics of EPS production were investigated. The coconut water medium was found to support the production of both types of EPS. The optimal initial pH and temperature was found to be 6·0 and 30°C, respectively. In shake flask (150 rev min −1 ) studies, high‐cell density inoculum resulted in the production of 11·50 g l −1 of WIS‐EPS and 4·01 g l −1 WS‐EPS after 72 and 96 h of fermentation, respectively. Conclusions: Coconut water was found suitable for the production of microbial EPS by Agrobacterium sp. CFR 24 strain. Under optimum conditions, it produced a good amount of WIS‐EPS, which is comparable with that of the sucrose medium (11 g l −1 ). Significance and Impact of the Study: This is the first report on the use of coconut water as a fermentation medium for the production of any microbial EPS. Besides producing value‐added products, use of this food industry byproduct, which is often being drained out, can significantly reduce the problem of environmental pollution.