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Laccase activity from the fungus Trametes hirsuta using an air‐lift bioreactor
Author(s) -
Rodríguez Couto S.,
Rodríguez A.,
Paterson R.R.M.,
Lima N.,
Teixeira J.A.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2006.01879.x
Subject(s) - laccase , bioreactor , fungus , trametes versicolor , glycerol , food science , botany , chemistry , biology , enzyme , biochemistry
Aim: To produce high laccase activities from the white‐rot fungus Trametes hirsuta in an in‐house air‐lift bioreactor (ALB). Methods and Results: Trametes hirsuta was grown in a 6‐l ALB. A fed‐batch strategy with glycerol as an addition resulted in maximum laccase activity of 19 400 U l −1 , which was the highest reported from the fungus. Conclusion: The ALB configuration with additional glycerol resulted in high laccase activities. Significance and Impact of the Study: This study provides useful information on how to produce high concentrations of laccase.