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Differentiation of outer membrane proteins from Salmonella enterica serotypes using Fourier transform infrared spectroscopy and chemometrics
Author(s) -
Kim S.,
Kim H.,
Reuhs B.L.,
Mauer L.J.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01828.x
Subject(s) - chemometrics , fourier transform infrared spectroscopy , serotype , salmonella , linear discriminant analysis , infrared spectroscopy , chromatography , chemistry , bacterial outer membrane , fourier transform spectroscopy , analytical chemistry (journal) , biology , microbiology and biotechnology , bacteria , mathematics , biochemistry , physics , escherichia coli , organic chemistry , quantum mechanics , genetics , statistics , gene
Abstract Aims: To differentiate between outer membrane proteins (OMPs) from six Salmonella enterica serotypes using a Fourier transform infrared (FTIR) spectroscopy method and chemometrics. Methods and Results: The OMPs from Salmonella serotypes (Typhimurium, Enteritidis, Thomasville, Hadar, Seftenberg and Brandenburg) were isolated using a sarcosyl extraction method. OMP profiles on SDS‐PAGE exhibited two or three bands between 48 and 54 kDa. Spectra of 10 μ l of OMP preparations (5 mg ml −1 ) dried on a gold reflective slide were collected using 128 scans at 4 cm −1 resolution and units of log (1/R) and analyzed using canonical variate analysis (CVA) and linear discriminant analysis (LDA). The CVA of Salmonella OMP spectra in the 1800–1500 cm −1 region separated the serotypes and LDA provided a 100% correct classification. Conclusions: The use of a FTIR method combined with chemometrics provided better differentiation of Salmonella OMPs than the OMP pattern analysis by SDS‐PAGE. Significance and Impact of the Study: This is the first study to demonstrate that spectra of OMP extracts from Salmonella serotypes can be used for 100% correct classification of the serotypes studied.