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Disruption of ste 23 gene affects composition profile and bioactivity of exopolysaccharide produced by Streptomyces sp. 139
Author(s) -
Zhang T.,
Wang L.,
Xu G.,
Chen Y.,
Zhang Y.,
Li Y.
Publication year - 2006
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01810.x
Subject(s) - beijing , china , chinese academy of sciences , library science , zhàng , traditional medicine , political science , medicine , law , computer science
Aims: To study the function of the gene ste 23 involved in the biosynthesis of Ebosin. Methods and Results: In search of databases, the deduced product of the gene ste 23 showed high homology to dTDP‐4‐dehydrorhamnose 3,5‐epimerases. ste 23 was replaced by a kanamycin resistance gene through double crossover. Compared with Ebosin, an exopolysaccharide (EPS) produced by wild‐type Streptomyces sp. 139, the EPS produced by the ste 23 mutant (designated EPS1) had a remarkably different monosaccharide composition and significantly diminished rhamnose content, though the molecular mass of EPS1 was similar to that of Ebosin. In addition, EPS1 lost the interleukin 1 (IL‐1) antagonist activity in vitro . Conclusions: ste 23 may be involved in the Ebosin biosynthesis in S. sp. 139. and its bioactivity. Significance and Impact of the Study: This is the first genetic work investigating functions of genes involved in EPS production in streptomyces by gene replacement of the pathway genes.