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Bifidobacterium ‐selective isolation and enumeration from chicken caeca by a modified oligosaccharide antibiotic‐selective agar medium
Author(s) -
Thitaram S.N.,
Siragusa G.R.,
Hinton A.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01765.x
Subject(s) - bifidobacterium , microbiology and biotechnology , bifidobacterium longum , lactobacillus acidophilus , biology , actinomycetaceae , agar , lactobacillus , bifidobacterium animalis , bacteria , probiotic , food science , fermentation , genetics
Aims: To determine the efficacy and selectivity of an acidified, antibiotic‐selective, oligosaccharide‐containing media for enumerating Bifidobacterium spp. from chicken caeca samples. Methods and Results: Transoligosaccharide propionate agar medium (TOS) modified by addition of mupirocin (50 μ g ml −1 ) and glacial acetic acid (1%, v/v), did not inhibit the growth of bifidobacteria compared with the control media yet inhibited the growth of Lactobacillus acidophilus , Lactobacillus gallinarum , Lactobacillus helveticus and Streptococcus gordonii . Conclusions: Addition of mupirocin (50 μ g ml −1 ) and glacial acetic acid (1%, v/v) to TOS (TOS‐AM50), is an effective selective medium for isolation and enumeration of Bifidobacterium spp. from chicken caeca samples. Significance and Impact of the Study: The development of an intestinal bifidobacteria‐selective media contributes to the study of probiotics and prebiotics in poultry and potentially other species.