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Effect of media, temperature and culture conditions on the species population and antibiotic resistance of enterococci from broiler chickens *
Author(s) -
Jackson C.R.,
FedorkaCray P.J.,
JacksonHall M.C.,
Hiott L.M.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01749.x
Subject(s) - enterococcus faecium , enterococcus faecalis , enterococcus , agar , biology , microbiology and biotechnology , antibiotics , population , broiler , antibiotic resistance , bacteria , incubation , agar plate , food science , staphylococcus aureus , medicine , biochemistry , genetics , environmental health
Aims: The effect of media type, incubation temperature and enrichment period on the species population and antibiotic susceptibility of enterococci from poultry carcass rinsates was determined. Methods and Results: Aliquots of rinsates, incubated in BBL Enterococcosel broth at 37°C, 42°C, or 45°C for 24 and 48 h, were inoculated onto BBL Enterococcosel and M‐enterococcus agar. Presumptive positive colonies were identified to species and tested for antibiotic resistance. Significant differences ( P ≤ 0·05) were observed for media and temperature. More Enterococcus faecalis were isolated from M‐enterococcus media and at 37°C while more E. faecium were isolated from Enterococcosel agar and at 45°C. The number of antibiotic‐resistant E. faecalis and E. faecium were also affected by media and temperature. Conclusions: Culture conditions for enterococci affect the observed species and antibiotic resistance patterns and therefore should be carefully considered. Significance and Impact of the Study: This study indicates that media and temperature can influence the recovery and selection of enterococcal species and antibiotic susceptibility.