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Multicomponent phenol hydroxylase‐catalysed formation of hydroxyindoles and dyestuffs from indole and its derivatives
Author(s) -
Kim J.Y.,
Kim J.K.,
Lee S.O.,
Kim C.K.,
Lee K.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01734.x
Subject(s) - indole test , phenol , chemistry , enzyme , organic chemistry , stereochemistry
Aims: To establish multicomponent phenol hydroxylases (mPHs) as novel biocatalysts for producing dyestuffs and hydroxyindoles such as 7‐hydroxyindole (7‐HI) from indole and its derivatives. Methods and Results: We have isolated Pseudomonas sp. KL33, which possesses a phenol degradation pathway similar to that found in Pseudomonas sp. CF600. Pseudomonas sp. KL28 is a strain that can grow on n ‐alkylphenols as a carbon and energy source. Escherichia coli strains expressing mPH from strain KL28 (mPH KL28 ) and strain KL33 (mPH KL33 ) catalysed the formation of indigo and 7‐HI, respectively, from indole. In addition, both mPHs catalysed the production of dyestuffs and hydroxyindoles from indole derivatives. The mPH KL28 has proved to be one of the most versatile biocatalysts that can accommodate a wide range of indole derivatives for catalysing the formation of dyestuffs. Conclusions: The present work provides a new approach in producing various dyestuffs and hydroxyindoles from indole and its derivatives by mPHs. Significance and Impact of the Study: These results indicate that mPHs may serve as potential agents for organic syntheses as well as bioremediation.