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Species‐directed 16S rRNA gene nested PCR detection of Olsenella species in association with endodontic diseases
Author(s) -
Rôças I.N.,
Siqueira J.F.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01723.x
Subject(s) - 16s ribosomal rna , biology , nested polymerase chain reaction , root canal , ribosomal rna , asymptomatic , periapical abscess , gene , polymerase chain reaction , microbiology and biotechnology , genetics , pathology , medicine , dentistry
Aims: To investigate the occurrence of Olsenella uli and Olsenella profusa in samples taken from infections of endodontic origin using a devised 16S RNA gene‐based nested PCR protocol. Methods and Results: DNA extracted from clinical samples was initially amplified using universal 16S rRNA gene primers followed by a second round of amplification using the first PCR products to detect a specific fragment of either O. uli or O. profusa 16S rRNA gene. Olsenella uli was detected in 27% of samples from teeth with asymptomatic periradicular lesions and in 20% of pus samples taken from symptomatic (abscess) lesions. Olsenella profusa was detected in 7% of asymptomatic teeth and in 13% of symptomatic teeth. A new Olsenella phylotype was also detected. Conclusions: Olsenella species can take part in the microbiota associated with infections of the root canal of human teeth and a role in the pathogenesis of periradicular diseases is suspected. Significance and Impact of the Study: The present study demonstrates that molecular genetic methods keep expanding the list of candidate endodontic pathogens to include Olsenella species.