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A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines
Author(s) -
Lee H.B.,
Kim Y.,
Jin H.Z.,
Lee J.J.,
Kim C.J.,
Park J.Y.,
Jung H.S.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01719.x
Subject(s) - trichoderma harzianum , cytotoxicity , biology , hypocrea , hela , strain (injury) , cell culture , microbiology and biotechnology , biochemistry , botany , in vitro , genetics , enzyme , biological pest control , anatomy , trichoderma reesei , cellulase
Aims: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. Methods and Results: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH‐20 chromatography and HPLC. The chemical structures were determined by ESI‐ or HRFAB‐MS and 1 H and 13 C‐NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC 50 value of 0·65 and 5·07 μ g ml −1 respectively. Conclusions: Harzianum A with a chemical formula of C 23 H 28 O 6 was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines. Significance and Impact of the Study: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.